Scientific Calendar November 2023

Urinary tract infections cause significant workloads to diagnostic laboratories with up to 80% of the samples turning out to be UTI-negative. Classic UTI diagnostics is a long-lasting procedure, since urine cultures and antibiotic susceptibility tests have to be performed. The prescription of empiric antibiotics is often a consequence, fostering the rise of antimicrobial resistances [R32-1].

Various investigations on the analytical performance have demonstrated the potential of the UF-Series to rule-out UTI by the absence of bacteriuria with potential impact on workflows and microbiological workloads [e.g. R32-2]. Since an absence of bacteriuria is observed in the vast majority of suspected UTI specimen, this implies the absence of a bacterial UTI, eliminating the need for further diagnostic processing of these samples by urine culture.

Gilboe et al. [R32-3] have demonstrated a significant potential of a combination of the bacteria and leucocyte count on the rule-out of UTI. Here, around 42% of all UTI-suspected samples can be directly reported as negative on the day of sample reception (Fig.1, left branch).

In addition, the potential impact of the ‘BACT Info’ flag on improving workflows and reporting times has been investigated. Here, the performance of the ‘Gram negative?’ flag in this setting allowed for urine culture to be skipped and for a direct antibiotic susceptibility testing (AST) approach to be conducted. This shortened the reporting time for antibiotic susceptibility profiles (ASP) for UTI-positive samples with Gram negative bacteriuria. In total, for 53% of the UTI samples, an ASP can be reported on the day after sample reception (Fig. 1, centre branch).

Only for UTI-positive samples with Gram positive bacteriuria (‘Gram positive?’) or mixed populations (‘Gram pos/neg?’) the full diagnostic pathway including urine culture was required. This, however, only affects a minority of the suspected UTI cases and for only 5% of the samples can an ASP be reported two days after sample reception (Fig.1, right branch).

This investigation highlights the potential of the UF-Series to reduce urine culture workloads and to improve workflow efficiency and reporting times by fostering a rapid diagnostics approach.

Upper urinary tract infections are a result of the ascencion of the pathogens from the bladder up into the ureter and the kidneys. Depending on the progression of the upper urinary tract infection, the infection is further differentiated into ureteritis, pyelonephritis and nephritis. Though an upper UTI is not per se defined as a complicated UTI, the differentiation of upper and lower urinary tract infection is of clinical relevance, since renal involvement correlates with more severe complications and clinical outcomes [R33-1].

With 13 and 3 cases per 10,000 UTI cases in women and men, respectively, the incidence of upper UTI is less than lower UTI [R33-2] and coincide with additional and more severe symptoms, such as pain and discomfort in the flanks, lower back or around the genitals, a high temperature, shivering or chills, fatigue and diarrhoea [R33-3].

On the UF-Series, Oyaert et al. demonstrated the potential to differentiate between upper and lower urinary tract infection by utilisation of the RTEC parameter for the detection of renal tubular epithelial cells (Fig. 2). They concluded that in patients with a confirmed UTI, the presence of RTEC indicates an upper urinary tract infection. In these investigations, the diagnostic performance of this parameter outperformed classical markers of upper urinary tract infections, such as α1-microglobulin [R33-4].