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Chromogenic Method
Light of a specific wavelength is directed at the sample and reagent mixture and detected by a photodiode, where the transmitted light is converted into electrical signals. The change in the intensity of this transmitted light is used to determine the change in absorbance. This measurement is used for chromogenic assays like Antithrombin and Protein C where a chromogenic substrate in presence of an activator causes the reagent mixture to change colour.
