CyFlow Ploidy Analyser

Automatic Measurement of Ploidy Level and Genome Size

Screening for Haplo-, Diplo-, Triplo- and Polyploids
Ploidy and Genome size analysis in less than two minutes
Detection of Anisoploids, Allopolyploids and Aneuploids
No time consuming microscopic evaluation
Any plant material can be analysed: leaves, seedlings, roots, flowers, peel of fruits, seeds

High-performance flow cytometry

The CyFlow Ploidy Analyser is a compact flow cytometer for ploidy analysis, high resolution DNA and genome size analysis for plants, animals and micro- organisms.

This modular system is equipped with a UV LED (365nm) and/or a green laser ( 532nm) which, when used in combination with Partec CyStain reagents, provides high resolution DNA analysis with DAPI or propidium iodide.

The CyFlow Ploidy instrument measures the DNA content of many thousands of individual nuclei within seconds or minutes. The result is directly displayed as a DNA histogram on the internal 15.4” colour display. The automated, real-time ploidy analysis is extremely time- and cost- efficient.

Technical Specifications

Light sources	Up to two light sources UV LED (365 nm) Nd-YAG green laser at 532 nm (30 mW)
Optics	Standard set-up and filters for propidium iodide (long pass filter 590 nm) and/or DAPI/SSC (long pass filter 435 nm)
Flow system	Quartz flow cuvette for laminar sample transport and hydrodynamic focussing True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement Completely closed fluidic system Sample port with biosafety cleaning system
Software	Windows™-based FCM software CyView for real-time data acquisition, real-time data analysis and real-time data display Easy set-up of experimental templates (configuration files) Flow Cytometry Standard (FCS) data format for storage of original and evaluated data Peak analysis function
Dimensions	Stand-alone instrument: W 385mm x H 290mm x D 280mm With Autoloading Station: W 745mm With screen open: H 528mm